Transcriptomic changes of BrPP5 overexpressing transgenic lines

Plant phosphoprotein phosphatases (PPPs) are ubiquitous and multifarious proteins that respond to developmental requirements depending on stress and other signals from within and externals by its reversible protein dephosphorylation mechanisms. Herein, we investigate hitherto unknown functions of Brassica rapa protein phosphatase 5.2 (BrPP5.2) by overexpressing, phenotyping for heat stress responses and profiling the transcriptomic dynamics in transgenic B. rapa lines.植物磷酸蛋白磷酸酶（phosphoprotein phosphatases, PPPs）是一类广泛分布、功能多样的蛋白质，可通过可逆的蛋白质去磷酸化机制响应发育需求，并感知逆境及体内外各类信号。本研究通过对转基因芜菁（Brassica rapa）株系开展过表达、热胁迫响应表型鉴定及转录组动态谱分析，探究了芜菁蛋白磷酸酶5.2（BrPP5.2）迄今尚未阐明的功能。