Cannabis use is associated with potentially heritable widespread changes in autism candidate gene <i>DLGAP2</i> DNA methylation in sperm

Parental cannabis use has been associated with adverse neurodevelopmental outcomes in offspring, but how such phenotypes are transmitted is largely unknown. Using reduced representation bisulphite sequencing (RRBS), we recently demonstrated that cannabis use is associated with widespread DNA methylation changes in human and rat sperm. Discs-Large Associated Protein 2 (<i>DLGAP2)</i>, involved in synapse organization, neuronal signaling, and strongly implicated in autism, exhibited significant hypomethylation (p DLGAP2 for nine CpG sites located in intron seven (p DLGAP2 expression in human conceptal brain tissue were inversely correlated (p Dlgap2 in sperm (p

父母吸食大麻与子代不良神经发育结局存在关联，但此类表型的传递机制目前仍在很大程度上尚不明确。本研究团队此前通过简化代表性亚硫酸氢盐测序（reduced representation bisulphite sequencing, RRBS）证实，吸食大麻与人类及大鼠精子中广泛存在的DNA甲基化改变存在关联。盘状大蛋白相关蛋白2（Discs-Large Associated Protein 2, DLGAP2）参与突触组织、神经元信号传导过程，并与自闭症发病密切相关，其在相关位点呈现显著低甲基化（原文缺失p值相关统计数据）；针对第七内含子内9个CpG位点的（原文缺失统计检验数据）分析显示，DLGAP2在人类胚胎脑组织中的表达与（原文缺失关联变量）呈负相关，且与精子中Dlgap2的（原文缺失相关关联数据）存在关联。

搜集汇总

背景与挑战

背景概述

该数据集研究大麻使用与自闭症候选基因DLGAP2在精子中DNA甲基化的广泛变化之间的关联，发现人类和大鼠精子中多个CpG位点甲基化差异，并提示这些变化可能具有遗传性，影响后代神经发育。数据集包含实验数据、图表及资助信息，发布于2019年，涵盖生物化学、遗传学和神经科学等领域。