Replication Data for: Mecp2 Deficiency Impairs Microscale Cortical Network Topology and Dynamics in a Rett Syndrome Mouse Model

This Dataverse includes the replication data for \"Mecp2 Deficiency Impairs Microscale Cortical Network Topology and Dynamics in a Rett Syndrome Mouse Model.\" This study investigated the effect of a loss-of-function mutation in Mecp2 on the development of microscale functional neuronal networks in primary cortical cultures from a mouse model of Rett syndrome. ** Dataverse contents: ** Spike time series: Spike time series for 60-electrode microelectrode array (MEA) recordings from wildtype (WT) and heterozygous (HET) or hemizygous (KO) for a Mecp2 deletion (exon 3 and 4) murine 2D cortical cultures at days-in-vitro (DIV) 14, 21, 28, and 35. The MEA voltage time series were first bandpass filtered (third-order Butterworth filter, 600-8000 Hz). Template-based spike detection was performed using the bior1.5 template in MATLAB (MathWorks) with a cost parameter of -0.0627. Metadata for the spike time series: Genotype and DIV for each MEA recording is included in the .mat file (open in MATLAB) with the spike time series. Analysis code: MATLAB scripts used for the analysis of the MEA spike time series. Upload in progress.本数据集仓库（Dataverse）收录了题为《Mecp2缺陷损害雷特综合征小鼠模型的微观尺度皮层网络拓扑结构与动力学》的重复验证数据。本研究以雷特综合征小鼠模型的原代皮层培养物为对象，探究了Mecp2功能丧失型突变对微观尺度功能性神经元网络发育的影响。**数据集仓库内容：** 放电时间序列：涵盖60通道微电极阵列（microelectrode array, MEA）的放电时间序列数据，记录样本为体外培养天数（days-in-vitro, DIV）14、21、28及35天的小鼠原代二维皮层培养物，其基因型分为野生型（wildtype, WT）、携带Mecp2（外显子3和4）缺失的杂合型（heterozygous, HET）以及半合子型（hemizygous, KO）。首先对MEA电压时间序列实施带通滤波处理，采用三阶巴特沃斯滤波器（third-order Butterworth filter），滤波频段设定为600~8000 Hz。基于模板的放电检测环节采用MATLAB（MathWorks）内置的bior1.5模板完成，代价参数设置为-0.0627。 放电时间序列元数据：每一次MEA记录对应的基因型与体外培养天数信息，均与放电时间序列一同存储于.mat文件中，可通过MATLAB直接打开。 分析代码：用于MEA放电时间序列分析的MATLAB脚本文件。 上传进行中。