Genetic Fate Mapping of Transient Cell Fate Reveals N-Cadherin Activity and Function in Tumor Metastasis

We obtained mice with multiple genotypes through mouse mating, and treated mice with tamoxifen at specified time points according to the experimental purpose, so as to make recombinase Cre enter the nucleus and produce homologous recombination of Cre-loxP to drive the expression of subsequent gene reporter genes to label the cells of interested. And trace the fate of these labeled cells in vivo. During the experiment, we randomly divided the experimental mice and the control group into three to five biological replicates in each group. We used Zeiss (V16) fluorescence microscope to carry out fluorescence imaging of whole tissue samples. The frozen section specimens were prepared by Thermo frozen slicer. Olympus (FV1200) and Nikon (A1) confocal microscopes were used for fluorescence imaging of tissue sections. BD flow cytometry was used for cell analysis and sorting. The StepOnePlus real-time PCR System (Applied Biosystems) was used for cDNA amplification. Image experimental results were analyzed using Image J. The data experimental results were analyzed using Prism.