pRS313, pRS314 and YCp50 are centromeric vectors while pRS424 is a 2μ vector [75, 76].TDH3 and PRC1 promoters are strong and moderate constitutive promoters, respectively. All plasmids harbor ACT1 ter
Table S1. Primers used in this study and detailed plasmid construction. Primers used for creating the S. pombe and P. pastoris plasmids are separated. Also primers for construction of the plasmids are
Asterisks (*) indicate previously existing Biobricks. “Registry” refers to the Registry reference, whereas “GenBank” refers to the GenBank accession number.
