Additional file 5: of Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris
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Table S1. Primers used in this study and detailed plasmid construction. Primers used for creating the S. pombe and P. pastoris plasmids are separated. Also primers for construction of the plasmids are separated from primers for sequencing and insertion of GOIs. In addition separate spreadsheets are providing information on the exact construction of the plasmids by listing the PCR products and restriction enzymes used for assembly.
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2017-12-19
