Study of key RNA metabolism proteins in <i>Enterococcus faecalis</i>

The control of mRNA turnover is essential in bacteria to allow rapid adaptation, especially in opportunistic pathogen like <i>Enterococcus faecalis</i>. This mechanism involves RNase and DEAD-box helicases that are key elements in RNA processing and their associations form the degradosome with accessory proteins. In this study, we investigated the function of four RNases (J1, J2, Y and III) and three DEAD-box helicases (CshA, CshB, CshC) present in most Enterococci. The interactions of all these RNA metabolism actors were investigated <i>in vitro</i>, and the results are in accordance with a degradosome structure close to the one of <i>Bacillus subtilis</i>. At the physiological level, we showed that RNase J1 is essential, whereas RNases J2 and III have a role in cold, oxidative and bile salts stress response, and RNase Y in general fitness. Furthermore, RNases J2, Y and III mutants are affected in virulence in the <i>Galleria mellonella</i> infection model. Concerning DEAD-box helicases, all of them are involved in cold shock response. Since the Δ<i>cshA</i> mutant was the most stress impacted strain, we studied this DEAD-box helicase CshA in more detail. This showed that CshA autoregulates its own expression by binding to its mRNA 5ʹUnstranslated Region. Interestingly, CshC is also involved in the expression control of CshA by a hitherto unprecedented mechanism.