Achilles dataset (version 21Q2), containing sgRNA-level fold-changes and pre-processed with CRISPRcleanR for the correction of gene-independent response to CRISPR-Cas9 targeting
Crystal structure of Mn-bound S.pyogenes Cas9 Descriptor: CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1, MANGANESE (II) ION, SULFATE ION Authors: Jinek, M, Jiang, F, Taylor, D.W, Sternberg, S.H, Kaya, E, M
High-density oligo libraries and CRISPR-Cas9 technology have revolutionized large-scale genetic screens, primarily in cell culture. Here, we present novel platforms for multicellular systems that util
Table S1. Sequences of reagents used in the study. The table shows the sequences of the oligonucleotides and lssDNA donors, primers and TaqMan assays employed in this study. LoxP sites (for all condit
