High-density oligo libraries and CRISPR-Cas9 technology have revolutionized large-scale genetic screens, primarily in cell culture. Here, we present novel platforms for multicellular systems that util

NIAID Data Ecosystem60

Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in E. coli through Transient Inhibition of Host RecA Activity

细菌基因组学

CRISPR-Cas9

Phage-derived “recombineering” methods are utilized for bacterial genome editing. Recombineering results in a heterogeneous population of modified and unmodified chromosomes, and therefore selection m

NIAID Data Ecosystem50

Additional file 1: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

基因组编辑

CRISPR-Cas9

Table S1. Sequences of reagents used in the study. The table shows the sequences of the oligonucleotides and lssDNA donors, primers and TaqMan assays employed in this study. LoxP sites (for all condit

Figshare2018-06-26 更新60

Table S2 from An In Vivo CRISPR Screen Identifies That SNRPC Promotes Triple-Negative Breast Cancer Progression

三阴性乳腺癌基因研究

CRISPR-Cas9

Table S2. sgRNA sequences of RBP genes in the CRISPR/Cas9 library.

Figshare2023-06-15 更新50