Transcriptomic changes in the pituitary when pituitary enhancer of the Nr5a1 gene is deleted

NR5A1 is expressed in the pituitary gonadotropes and regulates their functional differentiation. We have previously identified a pituitary-specific enhancer region located in the 6th intron of the Nr5a1 gene. In this study, deletion of the pituitary enhancer by genome editing abolished the expression of NR5A1 in the pituitary gland, confirming the functional importance of the enhancer. Transcriptomic changes in the enhancer-deleted mouse pituitary were revealed in both males and females.NR5A1在垂体促性腺细胞中表达，并调控其功能分化。我们此前已鉴定到位于Nr5a1基因第6内含子内的垂体特异性增强子区域。本研究通过基因组编辑技术敲除该垂体增强子后，垂体中NR5A1的表达完全消失，证实了该增强子的功能重要性。本研究同时揭示了增强子敲除的雌雄小鼠垂体组织的转录组变化。