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2022-10-26
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A Modular Scaffold for Controlling Transcriptional Activation via Homomeric Protein–Protein Interactions
Protein–protein interactions (PPIs) have been extensively utilized in synthetic biology to construct artificial gene networks. However, synthetic regulation of gene expression by PPIs in E. coli has l
Figshare80
High-throughput DNA engineering by mating bacteria
To reduce the operational friction and scale DNA engineering, we developed an in vivo DNA assembly technology platform called SCRIVENER. SCRIVENER combines bacterial conjugation, in vivo DNA cutting,
NIAID Data Ecosystem60
Data from: A self-transmissible plasmid from a hyperthermophile that facilitates genetic modification of diverse Archaea
Conjugative plasmids (CP) are self-transmissible mobile genetic elements which transfer DNA between host cells via Type IV Secretion Systems (T4SS). While T4SS-mediated conjugation has been well-studi
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Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
Control elements of the tetracycline-resistance operon encoded in Tn10 of Escherichia coli have been utilized to establish a highly efficient regulatory system in mammalian cells. By fusing the tet re
PubMed Central1992-06-15 更新30
