To reduce the operational friction and scale DNA engineering, we developed an in vivo DNA assembly technology platform called SCRIVENER. SCRIVENER combines bacterial conjugation, in vivo DNA cutting,
A recently published research article reported that the extreme halophile archaebacterium Natronobacterium gregoryi Argonaute enzyme (NgAgo) could cleave the cellular DNA under physiological temperatu
(A) Gel electrophoresis of NGS-derived PCR amplicons shows large deletions of POLR2J4 in clones #6 and #7 samples. (B) POLR2J4 indel rate. M indicates the normal cell line. #6 indicates a cell line wi
