Distribution of IFN-γ+ TEFF cells.
收藏资源简介：
Mice received naïve gBT-I or gDT-II cells prior to HSV-1 skin infection. (A) Quantification of TEFF cells in epidermis (Epi) and dermis (Der) 5–10 days post-infection, tracked as CD45.2+CD45.1+ Vα2+ (gBT-I) or Vα3.2+ (gDT-II) cells (dispase digestion). Data pooled from n = 9–14 mice/time point. (B,C) Analysis of IFN-γ+ TEFF cells in skin (dispase digestion) 5 days post-infection. **, Pn = 5–6 mice/group from 3–4 experiments. (D) Analysis of IFN-γ+ TEFF cells 5–10 days post-infection; n = 4–7 mice/time point from 1–4 experiments. (E,F) Analysis of IFN-γ+ TEFF cells in skin (gBT-I, epidermis; gDT-II, total skin), brachial (brLN) and axillary (axLN) LNs and spleen (5 days post-infection in E, or as indicated in F); n = 2–7 mice/organ/time point from 1–4 experiments.
