Green is RNA FISH using a probe against XIST RNA. Red is fluorescently labelled Cot-1. Blue is DAPI. These images were used to calculate data shown in Table 1.
We present Barcoded Oligonucleotides Ligated On RNA Amplified for Multiplexed and parallel In Situ analyses (BOLORAMIS), a reverse transcription-free method for spatially-resolved, targeted, in situ R