基于CRISPR/Cas12a的超灵敏MicroRNA检测纸基条带

基于链置换等温扩增及CRISPR-Cas12a信号放大技术，可研制超灵敏便携的miRNA金纳米检测试纸条。数据集来自于新型发光检测技术研究，其中包含一种基于CRISPR/Cas12a切割发光的超灵敏MicroRNA检测方法的数据。实验研究中，靶标 miR-31可以激活ISAR生成大量DNA，进一步触发 Cas12a切割以催化非特异性单链DNA切割，从而产生肉眼信号和荧光信号。所述数据通过多功能分子成像系统（Azure C600）采集获得，采集时间为2020年2月15日，采集地点为天津大学。数据体量约为50 kB。Based on strand displacement isothermal amplification and CRISPR-Cas12a signal amplification technologies, an ultrasensitive and portable gold nanoparticle-based test strip for miRNA detection can be developed. This dataset originates from research on novel luminescence detection technologies, and encompasses data for an ultrasensitive MicroRNA detection method utilizing CRISPR/Cas12a-mediated cleavage-induced luminescence. In the experimental study, the target miR-31 activates ISAR to generate large quantities of DNA, which further triggers Cas12a cleavage to catalyze non-specific single-stranded DNA cleavage, thereby producing both visual and fluorescent signals. The data were collected using a multifunctional molecular imaging system (Azure C600) on February 15, 2020, at Tianjin University. The total data volume is approximately 50 kB.

搜集汇总

背景与挑战

背景概述

该数据集包含基于CRISPR/Cas12a技术和链置换等温扩增的超灵敏MicroRNA检测方法数据，主要用于口腔鳞癌相关唾液miRNA的检测。数据通过多功能分子成像系统采集，体量约为50 kB，采集于2020年2月15日的天津大学。