Quantification Figure 3 (GraphPad Prism file)

Cytokines and BDNF secretion from human iPSC-derived astrocytes. Conditioned media were collected after stimulating cells during 24 h with 10 ng/mL TNF-α. Cytokines and BDNF secretion was measured in the conditioned media and compared with cells treated with vehicle. (a) - Pro-inflammatory cytokines: Interleukin-1 beta (IL-1β), Interleukin-8 (IL-8), Interferon gamma (IFN-γ) and Tumor necrosis factor alpha (TNF-α); (b) - modulatory cytokines: Interleukin-2 (IL-2), Interleukin-4 (IL-4) and Interleukin-6 (IL-6); (c) - anti-inflammatory cytokines Interleukin-10 (IL-10), Interleukin-13 (IL-13) and Brain-derived neurotrophic factor (BDNF). Data are presented as means ± SEM of concentrations in (pg/ml) of secreted factors. Conditioned media were collected from 4 cell lines and the experiments were performed in duplicates. *P < 0.05; **P < 0.01; ***P < 0.001; ns - non-significant. Unpaired Student´s t-test.

人诱导多能干细胞（induced pluripotent stem cell, iPSC）来源的星形胶质细胞所分泌的细胞因子与脑源性神经营养因子（Brain-derived neurotrophic factor, BDNF）。将细胞以10 ng/mL的肿瘤坏死因子α（Tumor necrosis factor alpha, TNF-α）刺激24小时后收集条件培养基，检测条件培养基中的细胞因子与BDNF分泌水平，并与溶剂处理的细胞组进行对照比较。
(a) 促炎细胞因子：白细胞介素-1β（Interleukin-1 beta, IL-1β）、白细胞介素-8（Interleukin-8, IL-8）、干扰素γ（Interferon gamma, IFN-γ）与肿瘤坏死因子α（TNF-α）；
(b) 调节性细胞因子：白细胞介素-2（Interleukin-2, IL-2）、白细胞介素-4（Interleukin-4, IL-4）与白细胞介素-6（Interleukin-6, IL-6）；
(c) 抗炎细胞因子：白细胞介素-10（Interleukin-10, IL-10）、白细胞介素-13（Interleukin-13, IL-13）与脑源性神经营养因子（BDNF）。
数据以分泌因子浓度的均值±标准误（standard error of the mean, SEM）表示，单位为pg/mL。条件培养基取自4株细胞系，所有实验均设置两次重复。*P < 0.05；**P < 0.01；***P < 0.001；ns 代表无统计学显著性差异。采用非配对Student t检验（Unpaired Student´s t-test）进行统计分析。