Quantification Figure 6 (GraphPad Prism file)

Impairment of [3H] D-aspartate uptake by TNF-α in human iPSC-derived astrocytes.Aspartate uptake was carried out (a) – 1 day or (b) – 5 days after exposing cells to vehicle or 10 ng/mL TNF-α. The competitive inhibitor of glutamate transporters DL-TBOA was added 10 minutes prior to aspartate. Data are presented as means ± SEM of the percentage of counts per minute (cpm). (c) – Cell viability was evaluated by ethidium incorporation. As a positive control for cell death, cells were lysed with Triton 2 %. As a positive control for cell viability, cells grown in DMEM/F12 with 10 % SFB were also evaluated. Data are presented as means ± SEM of the percentage of ethidium incorporation (arbitrary units of fluorescence). Data from 3 cell lines and experiments were performed in duplicates (a), (b) and quadruplicates (c). *P < 0.05; **P < 0.01; ***P < 0.001; One-way ANOVA followed by Tukey post hoc test. ns – Non-significant.

肿瘤坏死因子α（TNF-α）对人类诱导多能干细胞（induced pluripotent stem cells, iPSC）衍生星形胶质细胞摄取[³H]D-天冬氨酸的损伤作用。天冬氨酸摄取实验分别于细胞暴露于溶媒或10 ng/mL TNF-α后的（a）1天、（b）5天开展。谷氨酸转运体竞争性抑制剂DL-TBOA于天冬氨酸孵育前10分钟加入。数据以每分钟计数（counts per minute, cpm）的百分比均值±标准误（SEM）呈现。（c）通过乙啶掺入法评估细胞活力：以2%曲拉通（Triton）裂解细胞作为细胞死亡阳性对照，以添加10% SFB的DMEM/F12培养基培养的细胞作为细胞活力阳性对照，同步进行评估。数据以乙啶掺入率（荧光任意单位）的均值±标准误（SEM）呈现。本实验采用3株细胞系开展，其中（a）、（b）组实验设置2个复孔，（c）组实验设置4个复孔。*P < 0.05；**P < 0.01；***P < 0.001；统计方法采用单因素方差分析（One-way ANOVA）后进行Tukey事后检验。ns表示无显著性差异。