Blood DNA from up to 136 ARID patients were enriched in the targeted exons and deep sequenced. The aim is to unravel the variants in the specific linkage intervals and identify the causal mutations.
Forward and reverse primers were used for PCR amplification, internal primer was used for pyrosequencing. All forward primers for the promoter and exonic regions include a 5′-T7 sequence (5′-TAATACGAC
