*The FIP primer consisted of F2 and the complementary strand of F1 (F1c). **The BIP primer consisted of B2 and the complementary strand of B1 (B1c). F3 and B3; Outer primers, FIP and BIP; Inner primer
Forward and reverse primers were used for PCR amplification, internal primer was used for pyrosequencing. All forward primers for the promoter and exonic regions include a 5′-T7 sequence (5′-TAATACGAC