Tracking the Evolution of Emerging Influenza A Hemagglutinin Variants in IAV Populations under Selection

We have performed viral population sequencing of Hemagglutinin (HA) genomic RNA using the PCR/amplicon-based primerID technique or using standard shotgun (Nextera) sequencing. Samples selected for sequencing included an Influenza A Virus selected for twelve rounds of monoclonal antibody pressure, SV12. The SV12 parent strain and 3 parallel lines passaged through egg were selected for sequencing to determine the landscape of mutations acquired upon release of antibody pressure.