C57BL/6JGpt-H11^{em1Cin(Col1a1-iCre)}/Gpt

该品系在小鼠 Col1a1 启动子的作用下，在成骨细胞特异表达 Cre 酶。中靶小鼠可作为 成骨细胞诱导 LoxP 重组的 Cre 工具鼠。例如删除 FLOX 区域，即将该工具鼠与得到的条件 性敲除模型小鼠配繁，可在成骨细胞特异性删除两个 LoxP 之间的基因片段。 通过 Cas9 技术定点整合至 H11 位点，而 H11 位点作为 KI 的插入位点具有显著的优势： 1.转入的基因定点整合至染色体，故传代稳定，不会出现因传代导致表达丢失的现象；2.能 避免基因组上毗邻序列的干扰效应；3. 外源基因插入到这个位置不会破坏任何内源性基因， 并且小鼠生长发育正常、不影响小鼠表型及功能。 2023-3-22（刘珊珊）：DNA定量显示精子出现泄露。This mouse strain specifically expresses Cre recombinase in osteoblasts under the control of the mouse Col1a1 promoter. Targeted mice can serve as Cre driver mice for inducing LoxP recombination in osteoblasts. For example, to delete the FLOX region, this driver mouse can be crossbred with established conditional knockout mouse models, enabling the specific deletion of the gene fragment flanked by two LoxP sites in osteoblasts. The transgene is integrated site-specifically into the H11 locus via Cas9 technology, and the H11 locus has significant advantages as a knock-in (KI) insertion site: 1. The transgene is stably integrated into the chromosome, ensuring germline transmission stability without expression loss caused by consecutive passaging; 2. It avoids interference effects from adjacent genomic sequences; 3. Insertion of exogenous genes at this locus does not disrupt any endogenous genes, and mice develop normally without affecting their phenotypes or physiological functions. 2023-3-22 (Liu Shanshan): DNA quantification showed sperm leakage.