Dataset supporting "Inhibitory feedback from the motor circuit gates mechanosensory processing in <i>Caenorhabditis elegans</i>"

This is the dataset used in the <i>PLOS Biology</i> publication- Kumar S, Sharma AK, Tran A, Liu M, Leifer AM (2023) Inhibitory feedback from the motor circuit gates mechanosensory processing in <i>Caenorhabditis elegans</i>. PLoS Biol 21(9): e3002280. Previously published closed-loop high throughput optogenetic delivery system (Liu M, Kumar S, Sharma AK, Leifer AM (2022) A high-throughput method to deliver targeted optogenetic stimulation to moving <i>C</i>. <i>elegans</i> populations. PLoS Biol 20(1): e3001524) was used to track worms crawling on an agarose plate as well as deliver optogenetic stimulus to worms in real time depending on the current behavior state of the worms. In this work, worms were optogenetically stimulated in various behavior states such as forward, turn, and reverse. We investigated key reversal neurons such as AIZ, RIM, AIB, AVE, and AVA as well as the turning neurons RIV, SMB, and SAA.Datasets are arranged in folders with the corresponding figure name. For e.g. the folder 'figure_2_and_supplementary_figure_S4' contains the dataset used in generating figure 2 and supplementary figure S4. Each folder consists of .mat file for a worm strain and the corresponding behavior assay. For e.g. in 'figure_2_and_supplementary_figure_S4' folder, there is a .mat file named as 'raw_data_AIB_closed_loop_blue_stim.mat'. This file contains the data for optogenetic stimulation in a closed-loop behavior assay for the strain expressing an excitatory opsin in neuron AIB. The .mat file has the following information on each worm track: worm's velocity, centerline, ellipse ratio, optogenetic stimulus, etc. More details about each of the experiments carried out in this study can be found in Table 2 of the <i>PLOS Biology </i>manuscript.The MATLAB code used to analyze the data and generate figures presented in this work is available on GitHub at https://github.com/leiferlab/kumar-sensorimotor-integration.gitIf you use this dataset, please cite the following works in addition to citing the dataset itself:Kumar S, Sharma AK, Tran A, Liu M, Leifer AM (2023) Inhibitory feedback from the motor circuit gates mechanosensory processing in <i>Caenorhabditis elegans</i>. PLoS Biol 21(9): e3002280.Liu M, Kumar S, Sharma AK, Leifer AM (2022) A high-throughput method to deliver targeted optogenetic stimulation to moving <i>C</i>. <i>elegans</i> populations. PLoS Biol 20(1): e3001524.本数据集用于发表于《PLOS Biology》的研究论文：Kumar S、Sharma AK、Tran A、Liu M、Leifer AM于2023年发表的《运动环路的抑制性反馈调控秀丽隐杆线虫（Caenorhabditis elegans）的机械感觉加工》，PLoS Biol 21(9): e3002280。 本研究使用了此前发表的闭环高通量光遗传递送系统（closed-loop high throughput optogenetic delivery system），该系统对应的论文为Liu M、Kumar S、Sharma AK、Leifer AM于2022年发表的《针对移动秀丽隐杆线虫（Caenorhabditis elegans，简称C. elegans）群体的靶向光遗传刺激高通量递送方法》，PLoS Biol 20(1): e3001524。该系统可用于追踪琼脂平板上爬行的线虫，并可根据线虫当前的行为状态实时向其递送光遗传刺激。 本研究中，我们在线虫处于前进、转向、后退等多种行为状态时对其实施了光遗传刺激。我们对关键后退神经元（包括AIZ、RIM、AIB、AVE及AVA）以及转向神经元（包括RIV、SMB及SAA）开展了相关研究。 数据集以对应图片名称命名的文件夹进行组织。例如，文件夹"figure_2_and_supplementary_figure_S4"包含用于生成图2及补充图S4的数据集。每个文件夹中包含对应线虫品系与行为实验的.mat格式文件。例如在"figure_2_and_supplementary_figure_S4"文件夹中，存在名为"raw_data_AIB_closed_loop_blue_stim.mat"的文件，该文件存储了针对AIB神经元表达兴奋性视蛋白的线虫品系开展的闭环行为实验中的光遗传刺激相关数据。.mat文件中包含每条线虫轨迹的如下信息：线虫运动速度、躯体中线轮廓、躯体椭圆轴比、光遗传刺激参数等。 本研究中各项实验的详细信息可参见该《PLOS Biology》论文的表2。用于分析本研究数据并生成文中图表的MATLAB代码已上传至GitHub，地址为https://github.com/leiferlab/kumar-sensorimotor-integration.git。 若您使用本数据集，请在引用数据集本身的同时，引用以下两篇论文： 1. Kumar S, Sharma AK, Tran A, Liu M, Leifer AM (2023) Inhibitory feedback from the motor circuit gates mechanosensory processing in Caenorhabditis elegans. PLoS Biol 21(9): e3002280. 2. Liu M, Kumar S, Sharma AK, Leifer AM (2022) A high-throughput method to deliver targeted optogenetic stimulation to moving C. elegans populations. PLoS Biol 20(1): e3001524.