Computational promoter analysis.

A PSSM [13] representing the conserved signals for Fur recognition in Y. pestis was used for the prediction of Fur-box like sequences within the 300 bp upstream DNA regions of the major biofilm-required genetic loci hmsT, hmsHFRS, and YPO0450-0498. The diguanylate cyclase gene YPO0449 was located in the putative operon YPO0450-0498. δ, the minus numbers indicated the nucleotide positions upstream of translation start, and D and R represented the direct and reverse sequences, respectively.

本研究采用针对鼠疫耶尔森菌（Y. pestis）中Fur识别保守信号的位置特异性得分矩阵（Position-Specific Scoring Matrix，PSSM）[13]，对主要生物膜必需基因座hmsT、hmsHFRS以及YPO0450-0498的300 bp上游DNA区域内的类Fur盒序列开展预测。双鸟苷酸环化酶基因YPO0449位于推定操纵子YPO0450-0498内部。符号δ及负数值代表翻译起始位点上游的核苷酸位置，D与R分别对应正向序列与反向序列。