The Neuroanatomical Ultrastructure and Function of a Biological Ring Attractor - Kir perturbation calcium imaging data

All calcium imaging experiments that used Gal80 and the inwardly rectifying potassium channel Kir are contained in this zip file. The directories are arranged first by cell type, then by the date of the experiment for that cell type. Each trial has its own .mat files, and each mat file features a matrix containing the raw DF/F signal at each time point for each region of interest (ROIaveMax) and a structure that contains the behavioral data (positionDat). Analysis code can be found at https://github.com/DanTurner-Evans/BiologicalRingAttractor-CodeRelated materials in the collection (https://doi.org/10.25378/janelia.c.5179721):Neuron skeletons and synapses: https://doi.org/10.25378/janelia.12497756One color calcium imaging data: https://doi.org/10.25378/janelia.12490373Shi perturbation calcium imaging data: https://doi.org/10.25378/janelia.12490313Two color calcium imaging data: https://doi.org/10.25378/janelia.12490274Behavioral data for the perturbation experiments: https://doi.org/10.25378/janelia.12490070Dataset supports the publication:Turner-Evans, D. B., Jensen, K. T., Ali, S., Paterson, T., Sheridan, A., Ray, R. P., Wolff, T., Lauritzen, J. S., Rubin, G. M., Bock, D. D., & Jayaraman, V. (2020). The Neuroanatomical Ultrastructure and Function of a Biological Ring Attractor. Neuron, 108(1), 145-163.e10. https://doi.org/10.1016/j.neuron.2020.08.006AbstractNeural representations of head direction (HD) have been discovered in many species. Theoretical work has proposed that the dynamics associated with these representations are generated, maintained, and updated by recurrent network structures called ring attractors. We evaluated this theorized structure-function relationship by performing electron-microscopy-based circuit reconstruction and RNA profiling of identified cell types in the HD system of Drosophila melanogaster. We identified motifs that have been hypothesized to maintain the HD representation in darkness, update it when the animal turns, and tether it to visual cues. Functional studies provided support for the proposed roles of individual excitatory or inhibitory circuit elements in shaping activity. We also discovered recurrent connections between neuronal arbors with mixed pre- and postsynaptic specializations. Our results confirm that the Drosophila HD network contains the core components of a ring attractor while also revealing unpredicted structural features that might enhance the network’s computational power.本压缩包包含所有使用了Gal80以及内向整流钾通道Kir（inwardly rectifying potassium channel Kir）的钙成像实验数据。 目录结构首先按细胞类型分组，随后按对应细胞类型的实验日期进行排序。 每项实验对应独立的.mat格式文件，每个MAT文件均包含两类核心数据：其一为矩阵，存储了每个感兴趣区域（ROIaveMax）各时间点的原始DF/F信号；其二为结构体，包含行为学数据（positionDat）。 相关分析代码可通过以下链接获取：https://github.com/DanTurner-Evans/BiologicalRingAttractor-Code。 本数据集集合中的相关材料（DOI: 10.25378/janelia.c.5179721）包括： 1. 神经元骨架与突触数据：https://doi.org/10.25378/janelia.12497756 2. 单色钙成像数据：https://doi.org/10.25378/janelia.12490373 3. Shi扰动实验钙成像数据：https://doi.org/10.25378/janelia.12490313 4. 双色钙成像数据：https://doi.org/10.25378/janelia.12490274 5. 扰动实验行为学数据：https://doi.org/10.25378/janelia.12490070 本数据集支撑以下学术出版物：Turner-Evans, D. B., Jensen, K. T., Ali, S., Paterson, T., Sheridan, A., Ray, R. P., Wolff, T., Lauritzen, J. S., Rubin, G. M., Bock, D. D., & Jayaraman, V. (2020). 《生物环形吸引子的神经解剖超微结构与功能》. 神经元(Neuron), 108(1), 145-163.e10. https://doi.org/10.1016/j.neuron.2020.08.006 摘要 头部方向（head direction, HD）的神经表征已在诸多物种中被发现。理论研究提出，与这类表征相关的动态过程由被称为环形吸引子的循环神经网络结构生成、维持与更新。本研究通过基于电子显微镜的回路重建技术，以及对黑腹果蝇头部方向系统中已鉴定细胞类型的RNA测序分析，对该理论提出的结构-功能关联进行了验证。我们鉴定出了若干此前被推测的功能模块：包括在黑暗环境中维持头部方向表征、动物转向时更新该表征，以及将其锚定至视觉线索的相关模块。功能实验为单个兴奋性或抑制性回路元件在塑造神经活动中的预期作用提供了支持。我们还发现了兼具突触前与突触后特化结构的神经元分支间存在循环连接。本研究结果证实，黑腹果蝇的头部方向网络具备环形吸引子的核心组成要素，同时还揭示了可能增强该网络计算能力的、此前未被预测到的结构特征。