Gene expression in D8 WT and Menin KO uteri

Differential expression genes were normalized to fragments per kilobase of exon model per million mapped reads (RPKM) using EdgeR package in R with the criteria of fold change significantly greater than 1.5 and P<0.05. Gene ontology (GO) enrichment analysis of the DEGs was analyzed by using DAVID (the Database for Annotation, Visualization and Integrated Discovery, https://david.ncifcrf.gov). GO terms with corrected p <0.05 were considered significantly enriched. Gene Set Enrichment Analysis (GSEA) was performed to determine whether a defined set of genes shows statistically significant, consistent differences between Men1f/f and littermates Men1d/d decidual tissues. Total RNAs from D8 uteri were extracted by TRIzol and purified by poly-A before subjected to RNA-Seq. The expression of level of each gene is normalized to RPKM.差异表达基因（differentially expressed genes, DEGs）采用R语言中的EdgeR软件包进行标准化处理，以每百万映射读数的外显子模型每千碱基片段数（RPKM）作为标准化单位，筛选标准为倍数变化显著大于1.5且P值小于0.05。采用DAVID（数据库注释、可视化与整合发现数据库，https://david.ncifcrf.gov）对上述差异表达基因开展基因本体（Gene Ontology, GO）富集分析，以校正后P值小于0.05的GO条目视为显著富集条目。开展基因集富集分析（Gene Set Enrichment Analysis, GSEA），以明确特定基因集在Men1f/f与同窝出生的Men1d/d蜕膜组织之间是否存在统计学意义上的显著且一致的表达差异。提取第8天（D8）子宫组织的总RNA，采用TRIzol试剂进行总RNA提取，经poly-A尾纯化后进行RNA测序（RNA-Seq）；每个基因的表达水平均以RPKM进行归一化。