Comparison of hiPSCs, hESCs and fibroblasts

In our study, PRDM14 and NFRkB were found to enhance the reprogramming of human fibroblasts to hiPSCs in the presence of OCT4, SOX2, KLF4, with/without c-MYC (OSK/OSKC). To examnie if the obtained hiPSC share similar expression signature with hESC, we conducted the microarray analysis on the hiPSCs, hESCs and fibroblasts. The result showed that all of the examined hiPSCs resembled hESCs, but differed from fibroblasts MRC5. 4 hiPSC lines, 2 hESC lines and 1 type of fibroblasts were analyzed in total. Each sample is done in duplicates.本研究发现，PRDM14与NFRkB可在OCT4、SOX2、KLF4联合存在且辅以或不辅以c-MYC（即OSK/OSKC组合）的条件下，增强人成纤维细胞向人类诱导多能干细胞（human induced pluripotent stem cells, hiPSCs）重编程的效率。为探究所获得的hiPSCs是否与人类胚胎干细胞（human embryonic stem cells, hESCs）具有相似的表达谱特征，我们对hiPSCs、hESCs及成纤维细胞开展了基因芯片分析。结果显示，所有待测hiPSCs的表达谱均与hESCs相似，而与MRC5成纤维细胞存在显著差异。本研究共分析4株hiPSCs细胞系、2株hESCs细胞系以及1种成纤维细胞，所有样本均设置双重复实验。