Levels of miR-16 and miR-451 in different fractions of blood.

MiRNAs were isolated and quantified from either whole blood or different blood fractions separated by Ficoll gradient. Copy numbers per milliliter whole blood were calculated for each miRNA based on a standard curve generated using synthetic oligonucleotides with sequences corresponding to the mature miRNA sequence.本研究从全血或经Ficoll梯度离心分离得到的不同血液组分中分离并定量了微小核糖核酸（microRNA, miRNA）。针对每一种miRNA，研究人员以序列与成熟miRNA序列匹配的合成寡核苷酸制备标准曲线，据此计算得到每毫升全血中的拷贝数。