Dataset Genotypic diversity DENV-2 [67]

Genotypic diversity and molecular characterization of DENV-2 in a Peruvian endemic region from 2016 to 2022: displacement of American/Asian genotypeABSTRACTBackground: Dengue is a serious and lethal disease in the Asian and Latin American tropical and subtropical regions. It is a serious public health problem in Peru due to its hyperendemic nature as serotype 2 (DENV-2), aside from being the most prevalent serotype, causes the most serious clinical manifestations of the disease. This study focuses on the molecular characterization and intraserotypic diversity analysis of the DENV-2 that circulates in the endemic region of Cajamarca.Methods: Thirty-nine hundred sixty-seven (3,967) blood serum samples of patients with acute febrile illness (AFI) were analyzed between 2016 and 2022 to detect DENV and DENV-2 through real-time RT-PCR technique, and the viral envelope (E) gene was then sequenced by the Sanger method. Finally, the reconstruction of the phylogeny was performed using the maximum likelihood method."Results: Thirty-two (32) complete sequences of gene E were obtained in total, and the phylogenetic and characterization analysis of the amino acid sequence revealed that, during the 2016-2022 period, two DENV-2 genotypes, i.e., the Am/As genotype and the cosmopolitan genotype in their lineages 2 and C, respectively, circulated.Conclusion: Likewise, our findings showed that every studied outbreak was characterized by novel autochthonous variants in the case of the Am/As genotype and by an imported variant in the case of the cosmopolitan genotype; this, therefore, shows a temporal distribution of the intraserotypic variability that denotes the Am/As genotype displacement around 2021 and the rooting of the cosmopolitan genotype. The need of a constant genetic or genomic surveillance of the cosmopolitan virus arises in order to understand its distribution and diversification patterns in Peru.2016-2022年秘鲁流行地区登革病毒2型（DENV-2）的基因型多样性与分子特征：美洲/亚洲基因型的更替 摘要 背景：登革热在亚洲及拉丁美洲的热带、亚热带地区是一种严重且可致命的传染病。在秘鲁，登革热呈高度地方性流行态势，其中血清型2（DENV-2）不仅是最流行的血清型，还会引发该疾病最严重的临床表现，因此登革热已成为秘鲁严峻的公共卫生问题。本研究聚焦于卡哈马卡流行地区循环的登革病毒2型（DENV-2）的分子特征及血清型内多样性分析。 方法：本研究于2016-2022年间，对3967例急性发热性疾病（AFI）患者的血清样本进行分析，通过实时逆转录聚合酶链反应（real-time RT-PCR）技术检测登革病毒（DENV）及登革病毒2型（DENV-2）；随后采用桑格测序（Sanger）法对病毒包膜（E）基因进行测序。最终采用最大似然法（maximum likelihood method）进行系统发育重建。 结果：本研究共获得32条完整的包膜E基因序列。对氨基酸序列进行系统发育及特征分析后发现，2016-2022年间流行的登革病毒2型（DENV-2）存在两种基因型，分别为美洲/亚洲基因型（Am/As genotype）及其谱系2，以及全球流行基因型（cosmopolitan genotype）及其谱系C。 结论：本研究结果显示，美洲/亚洲基因型的每一次暴发均以新型本土变异株为特征，而全球流行基因型的暴发则伴随输入性变异株。上述结果表明血清型内变异的时间分布特征，具体表现为美洲/亚洲基因型在2021年左右发生更替，且全球流行基因型在秘鲁扎根。为明确该病毒在秘鲁的传播与演化模式，需对全球流行基因型病毒开展持续性的基因或基因组监测工作。