ChIP-Seq of constitutively expressed Ndt80-Myc in P. pastoris
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The purpose of this experiment was to identify the genes bound by Ndt80 in P. pastoris when constitutively expressed. Ndt80 was tagged with c-myc, the native Ndt80 promoter was replaced with the E. gossypii Tef1 promoter and the protein was immunoprecipitated with a c-myc antibody. Cells were grown unti log-phase in YPD. Each experiment was repeated twice and sequenced on an Illumina HiSeq 2500. P. pastoris Ndt80-myc vs. untagged control, with two technical replicates for each
创建时间：
2019-05-15
