Divergence time estimates (in Mya) for 15 cloud forest taxa that span the geographic barriers identified in this study for each of the taxa derived from the BEAST analyses.

Ranges correspond to 95% highest posterior density (HPD).Biogeographic affinity: NA = North America, SA = South America, CA = Central America.Geographic barrier: Isthmus of Tehuantepec (east and west of the isthmus), Los Tuxtlas (isolated from the Sierra Madre Oriental), Chiapas Central Depression (populations in Chiapas separated by the Central Depression), Isthmus 2 (the Chiapas and Los Tuxtlas populations separated from populations west of the Isthmus of Tehuantepec).各取值范围对应95%最高后验密度区间（Highest Posterior Density, HPD）。生物地理亲和性（Biogeographic Affinity）：NA=北美洲（North America），SA=南美洲（South America），CA=中美洲（Central America）。地理屏障（Geographic Barrier）：特万特佩克地峡（Isthmus of Tehuantepec，涵盖该峡东西两侧区域）、洛斯图斯特拉斯（Los Tuxtlas，与东马德雷山脉相互隔离）、恰帕斯中央洼地（Chiapas Central Depression，恰帕斯的种群被该洼地分隔）、地峡2（Isthmus 2，恰帕斯与洛斯图斯特拉斯的种群与特万特佩克地峡西侧的种群相互分隔）。