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Single cell RNA sequencing of HSC and MPP in DNA damage tolerant deficient mutants

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NIAID Data Ecosystem2026-03-14 收录
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We performed scRNA-seq according to the CEL-seq2 protocol in order to define transcriptome changes in mouse hematopoietic stem cell containing LSKs in different mutants with defects in DNA damage tolerances. Using FACS, we sorted 2-4 96 well plates for WT, Rev1-/-, PcnaK164R/K164R, and PcnaK164R/K164R;Rev1-/- LSK cells.

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2023-03-15
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