ExtFig3C_WB_Tubulin_293Tcells_R3_LG116.sgd

<em>Protein lysates were collected from A549 and HEKT293T ishXrn1 cells treated with doxycycline for 3-4 days or left untreated. They were then probed with antibodies against Xrn1 or beta-tubulin as a loading control, to check for efficient knock down of Xrn1. </em>收集经多西环素（doxycycline）处理3~4天或未处理的A549细胞与HEKT293T ishXrn1细胞的蛋白裂解液，随后使用靶向Xrn1的抗体以及作为上样对照的β微管蛋白抗体进行免疫检测，以验证Xrn1基因敲低的效率。