Acute exposure plasma parameters

To fully assess the impacts of Deepwater Horizon (DWH) oil on the vertebrate stress response and the trajectory for recovery, our research integrated molecular, cellular, gland/organ and whole animal physiological and behavioral studies using a benthic marine teleost, the Gulf toadfish, a resident of the Gulf of Mexico. These studies evaluated multiple pathways (e.g., pituitary fatigue, reduced receptor sensitivity, disruption or overstimulation of cortisol biosynthesis) and the consequence of these changes at the whole animal level (e.g., changes in carbohydrate metabolism, immune capacity and responses to natural stresses such as predation and social interaction). In this series of experiments, fish plasma parameters such as cortisol, adrenocorticotropic hormone (ACTH) and glucose concentrations were evaluated during short-term exposures to DWH oil. Each exposure was followed by a complementary recovery period (i.e., no oil or PAH exposure) to assess the recovery trajectory. These experiments were completed at Rosenstiel School of Marine and Atmospheric Science (RSMAS), University of Miami, Marine Technology & Life Sciences Seawater Complex (MTLSS) Building, room 211 between January 2018 to December 2018.为全面评估深水地平线（Deepwater Horizon, DWH）原油对脊椎动物应激反应的影响及其恢复轨迹，本研究以墨西哥湾常住底栖海洋硬骨鱼类——海湾豹蟾鱼为实验对象，整合了分子、细胞、腺体/器官及个体整体的生理与行为学研究。本研究评估了多条应激通路（如垂体疲劳、受体敏感性降低、皮质醇生物合成紊乱或过度刺激），并探究了上述变化在个体水平上的效应，具体包括碳水化合物代谢、免疫能力的改变，以及对捕食、社会互动等自然应激源的响应变化。在本系列实验中，研究人员在短期暴露于DWH原油的过程中，检测了鱼类血浆中的皮质醇、促肾上腺皮质激素（adrenocorticotropic hormone, ACTH）及葡萄糖浓度等生理参数。每次暴露后均设置了配套的恢复周期（即无原油或多环芳烃（Polycyclic Aromatic Hydrocarbons, PAH）暴露），以评估其恢复轨迹。本实验于2018年1月至2018年12月间，在迈阿密大学海洋与大气科学罗森斯蒂尔学院（Rosenstiel School of Marine and Atmospheric Science, RSMAS）海洋技术与生命科学海水综合楼（Marine Technology & Life Sciences Seawater Complex, MTLSS）211室完成。