Clean pyrosequencing data

These sequences were checked against the following quality criteria: (i) no Ns, (ii) quality score ≥ 23 according to the PANGEA process (Giongo et al., 2010), (iii) a minimum sequence length of 200 bp, and (iv) no sequencing error in the forward primer. The scripts used were quality.pl and preprocess.pl implemented in the pipeline PANAM (http://code.google.com/p/panam-phylogenetic-annotation /downloads /list) described more in detail in the related paper (Taib et al. 2013).本研究对下述序列开展质量校验，校验标准如下： （i）序列不含未知碱基（N）； （ii）基于PANGEA流程 (PANGEA process，Giongo等，2010) 的质量评分不低于23； （iii）序列最小长度为200个碱基对 (base pair)； （iv）正向引物区域无测序错误。 本次分析所使用的脚本为quality.pl与preprocess.pl，二者集成于PANAM系统发育注释分析流程 (PANAM phylogenetic annotation pipeline) 中；该流程的详细信息可参见其官方下载页面（http://code.google.com/p/panam-phylogenetic-annotation/downloads/list）及Taib等2013年发表的相关研究论文。