The effect of external flow on 3D orientation of a microscopic sessile suspension feeder, Vorticella convallaria

Vorticella convallaria are microscopic sessile suspension feeders that live attached to substrates in aquatic environments. They feed using a self‐generated current and help maintain the health of aquatic ecosystems and wastewater treatment facilities by consuming bacteria and detritus. Their environmental impact is mediated by their feeding rate. In ambient flow, feeding rates are highly dependent on an individual's orientation relative to the substrate and the flow. Here, we investigate how this orientation is impacted by flow speed. Furthermore, we examined whether individuals actively avoid orientations unfavorable for feeding. We exposed individuals to unidirectional laminar flow at shear rates of 0, 0.5, 1.0, and 1.5 s−1, and recorded their 3D orientation using a custom biplanar microscope. We determined that V. convallaria orientation became progressively tilted downstream as the shear rate increased, but individuals were still able to actively reorient. Additionally, at higher s..., Please see details in the methods section of T. Böttger, B. Klaassen van Oorschot, and R. E. Pepper, “The effect of external flow on 3D orientation of a microscopic sessile suspension feeder, Vorticella convallaria,” Annals of the New York Academy of Sciences, doi: 10.1111/nyas.15170., , # **Data and code from The effect of external flow on 3D orientation of a microscopic sessile suspension feeder, Vorticella convallaria**  These data consist of: 1\)    Matlab data files (.mat), which are raw data of angle vs. time for the cell body and stalk of 15 individuals. Details of the data files are described below. 2\)   plot_vorticella_angle_data.ipynb is a Python notebook that illustrates how to use the Matlab data files in Python if desired. 3\)   R code to do the statistical analyses described in our paper. This code contains further comments with details of each analysis. 4\)   Vorticella_data.xlsx. An Excel file that contains all data used in the R code in a format suitable for use with this code. Details of this data file are described below. 5\)  Flow channel laser cutter outlines.cdr and Flow channel laser cutter outlines.pdf. These files are to-scale outlines of the laser cut pieces used to make the flow chamber used in our experiments. The cored draw (.c...沟钟虫（Vorticella convallaria）是一类营固着生活的微型悬浮滤食生物，常附着于水生环境中的基质表面。它们依靠自身产生的水流获取食物，并通过摄食细菌与碎屑，助力维持水生生态系统及污水处理设施的健康运转。其生态影响由滤食速率所介导。在存在环境水流的条件下，滤食速率高度依赖于个体相对于基质与水流的朝向。本研究探究了水流速度对该朝向的影响，并进一步考察了个体是否会主动规避不利于摄食的朝向。实验中，我们将个体暴露于剪切率分别为0、0.5、1.0及1.5 s⁻¹的单向层流环境中，并通过定制的双平面显微镜记录其三维朝向。研究发现，随着剪切率升高，沟钟虫的朝向逐渐向水流下游倾斜，但个体仍可主动进行重定向。此外，在更高剪切率下……（原文未完成），详细方法参见T. Böttger、B. Klaassen van Oorschot与R. E. Pepper发表于《纽约科学院年鉴》的论文《外源水流对微型固着悬浮滤食生物沟钟虫（Vorticella convallaria）三维朝向的影响》，DOI: 10.1111/nyas.15170。 # **本数据集与代码源自论文《外源水流对微型固着悬浮滤食生物沟钟虫（Vorticella convallaria）三维朝向的影响》** 本数据集包含以下内容： 1) Matlab数据文件（.mat）：包含15个个体的虫体与柄部的角度-时间原始数据，数据文件的详细说明见下文。 2) plot_vorticella_angle_data.ipynb：一款Python交互式笔记本，用于演示如何在Python环境中调用上述Matlab数据文件（若有需要）。 3) 用于完成论文中所述统计分析的R代码，代码中包含各分析步骤的详细注释。 4) Vorticella_data.xlsx：一款Excel格式文件，包含了适配该R代码的全部实验数据，数据文件的详细说明见下文。 5) Flow channel laser cutter outlines.cdr与Flow channel laser cutter outlines.pdf：用于制作本实验所用流动腔的激光切割件的等比例轮廓文件，其中.cdr格式文件（原文未完成）