Mobility of condensin DC is key to its function in repressing transcription [RNA-seq II]

Analyze condensin binding by fluorescence recovery after photobleaching (FRAP) and chromatin immunoprecipitation followed by sequencing (ChIP-seq) in ATP hydrolysis mutant and in mutants that affect the level of histone H4K20 methylation and H4K16ac enrichment and depletion on the X chromosome. We have also performed Hi-C analysis to test how 3D DNA interactions mediated by condensin DC change in the catalytic and null mutant of dpy-21, a H4K20me2 demethylase that increase H4K20me1 on the X. Population mRNA profiles of mixed embryos with genotypes: WT, hsp::dpy-27::gfp or hsp::dpy-27[EQ-TR]::gfp.