光控外泌体的合成构建与白血病RNA药物递送数据集

肿瘤治疗过程中，如何温和、安全、靶向递送治疗剂至今仍是挑战。本研究利用合成生物学理念，创新性对外泌体的供体细胞进行基因再设计，发展了一种基于外泌体的光控RNA药物递送系统。该系统利用外泌体的生物起源以及RNA 适配体MS2-MCP蛋白相互作用、光诱导CIBN- CRY2蛋白之间的相互作用，实现了细胞内源 microRNA-21治疗剂（miR-21海绵）的高效装载和对白血病细胞的靶向递送。该研究为细胞表达的内源RNA药物提供了一种新的递送途径，并将助力更多RNA药物应用于肿瘤个性化治疗。Currently, the development of mild, safe and targeted delivery systems for therapeutic agents remains a critical challenge in cancer therapy. In this study, we leverage synthetic biology principles to innovatively genetically reprogram exosome donor cells, and develop an exosome-based light-controlled RNA drug delivery system. This system utilizes the biological origin of exosomes, the interaction between the RNA aptamer MS2 and MCP protein, as well as the light-induced interaction between CIBN and CRY2 proteins, to achieve efficient loading of the cellular endogenous microRNA-21 therapeutic agent (miR-21 sponge) and targeted delivery to leukemia cells. This study provides a novel delivery avenue for cell-expressed endogenous RNA therapeutics, and will facilitate the application of more RNA drugs in personalized cancer therapy.

搜集汇总

背景与挑战

背景概述

该数据集基于合成生物学理念，通过基因再设计构建了光控外泌体系统，用于高效装载和靶向递送细胞内源microRNA-21治疗剂至白血病细胞。它为RNA药物递送提供了一种新途径，有助于推动肿瘤个性化治疗的应用。