RNA-sequencing of estradiol inducible DOF6 overexpression (DOF6ioex) and rgl2-13 DOF6ioex seeds imbibed in mock or 50µM estradiol for 8h. RNA-sequencing of estradiol inducible DOF6 overexpression (DOF6ioex) and rgl2-13 DOF6ioex seeds imbibed in mock or 50µM estradiol for 8h

The DELLA protein RGA-LIKE2 (RGL2) is a key transcriptional repressor of gibberellic acid (GA) signaling that regulates seed germination.RGL2 lacks a DNA binding domain and requires a transcription factor to induce GATA12 expression. Our data show that this RGL2-containing protein complex includes DNA BINDING1 ZINC FINGER6 (DOF6), which is a known negative regulator of germination in freshly harvested seeds. The RGL2-DOF6 complex regulates primary seed dormancy in Arabidopsis by regulating downstreming targets. Overall design: Differential gene expression in freshly harvested DOF6ioex and rgl2-13 DOF6ioex seeds imbibed in mock or 50µM estradiol at room temperature for 8h