Isolation of murine hepatic myeloid cells with high yield and purity using immunomagnetic beads for subset analysis.

There are numerous established techniques for isolating hepatic myeloid cells; however, preserving their phenotypic and functional characteristics can be challenging. We present a straightforward and efficient method to isolate hepatic myeloid cells, including Kupffer cells and lymphocyte antigen 6 complex, locus C+ (Ly6C+) monocytes/macrophages. The procedure involves perfusion of the liver with collagenase and purification with immunomagnetic particles. This protocol ensures the isolation of large quantities of purified, viable, and functional cells without influencing their physiological characteristics. For complete details on the use and execution of this protocol, please refer to Wu et al. (2019).目前已有多种成熟的肝脏髓系细胞分离技术，但保留其表型与功能特性仍颇具挑战。本研究提出一种简便高效的肝脏髓系细胞分离方法，可分离得到库普弗细胞（Kupffer cells）以及淋巴细胞抗原6复合物位点C阳性（Ly6C⁺）单核细胞/巨噬细胞。该流程包括使用胶原酶对肝脏进行灌注，并借助免疫磁珠进行纯化。本实验方案可获得大量纯化、存活且功能完整的细胞，且不会影响其生理特性。如需了解本方案的使用与完整操作细节，请参阅Wu等人（2019）的研究。

搜集汇总

背景与挑战

背景概述

该数据集描述了一种使用免疫磁珠高效分离小鼠肝脏髓系细胞（包括Kupffer细胞和Ly6C+单核细胞/巨噬细胞）的实验方法。该方法通过胶原酶灌注和免疫磁珠纯化步骤，能够在保持细胞表型和功能特性的前提下，获得高产量和高纯度的细胞，适用于亚群分析。