Multiplex identifiers (MIDs) are underlined. Forward primers use MID7 and reverse primers use MID4 (Roche). Additional complementarity between RT-PCR primers and 454-Adapter PCR primers is identified
Underlined regions indicate Illumina adapter sequences. Bold-face font indicates PCR primer region, which is preceded by a linker sequence. Poly-N string in forward primer denotes barcode sequence.Bar
