贝莱斯芽孢杆菌分子鉴定与培育流程数据集

采用通用引物27F和1492R对菌株16S rDNA基因进行PCR扩增；运用BLAST算法在NCBI数据库对测序获得的核苷酸序列进行同源性比对，选择相似性不小于99%的序列作为参考；使用MEGA软件中的邻接法（N-J法）构建系统发育树，分析菌株进化地位。最终依据系统发育树分析结果与生理生化特征，从目标属（芽孢杆菌属）中选取目标种（贝莱斯芽孢杆菌），并通过标准化固体平板培养方法进行纯化扩增，得到目标菌株。The 16S rDNA gene of the strain was amplified via PCR using the universal primers 27F and 1492R. The nucleotide sequences obtained from sequencing were subjected to homology alignment against the NCBI database using the BLAST algorithm, and sequences with a similarity of no less than 99% were selected as reference sequences. A phylogenetic tree was constructed using the neighbor-joining (N-J) method in MEGA software to analyze the evolutionary status of the strain. Finally, based on the results of the phylogenetic tree analysis and physiological and biochemical characteristics, the target species (*Bacillus velezensis*) was selected from the target genus (*Bacillus*), and purified and amplified via the standardized solid plate culture method to obtain the target strain.

搜集汇总

背景与挑战

背景概述

该数据集聚焦于贝莱斯芽孢杆菌的分子鉴定与培育流程，规模为11KB，属于静态数据。它通过16S rDNA序列分析、系统发育树构建和标准化培养方法，为微生物分类学和工业菌种管理提供从鉴定到纯种制备的完整数据支持，适用于精准分子鉴定和规模化生产场景。