Baseline oxygen consumption decreases with cortical depth: Artery02_010819_VG674

The MathWorks Matlab files (‘.mat’) are named after the cortical depth and contain Oxyphor2P decay data (‘Oxyphor2P.decay’), the derived pO2 values (‘Oxyphor2P.pO2Value’) and their coordinates (X: ‘Oxyphor2P.X_reference _coordinates’ and Y: ‘Oxyphor2P.Y_reference _coordinates’), which relate to the reference images with astrocyte-staining with SR101 (‘reference.SR101’), vasculature containing FITC (‘reference.FITC’) and the Oxyphor2P distribution (‘reference.Oxyphor2P’). DATASET METADATA: title:'Baseline oxygen consumption decreases with cortical depth: Artery02_010819_VG674', Other:'Phosphorescence Lifetime Oxygen Imaging' SPECIMEN METADATA: LocalID:'VG674', Species:'Mouse', NCBITaxonomy:'NCBI:txid10090', Age:'212.0', Ageunit:'Days', Sex:'Female', Genotype:'C57BL6', OrganName:'Brain', SampleLocalID:'Artery02_010819_VG674', Locations:'somatosensory cortex' INSTRUMENT METADATA: MicroscopeType:'Two Photon Laser Scanning Microscopy', MicroscopeManufacturerAndModel:'Bruker Ultima', ObjectiveName:'Olympus UMPlanFL N, Olympus XLUMPlanFl', ObjectiveImmersion:'Water', ObjectiveNA:'0.5, 0.95', ObjectiveMagnification:'20x', DetectorType:'PMT', IlluminationTypes:'Chameleon Ultra femtosecond Ti:Sapphire laser (Coherent)' IMAGE METADATA: xAxis:'posterior-to-anterior', yAxis:'left-to-right', zAxis:'superior-to-inferior', Number:'1, 2, red, green', displayColor:'(1.0, 0.0, 0.0),(0.0, 1.0, 0.0)', Representation:'1: Oxyphor 2P; 2: FITC,', Flurophore:'Oxyphor2P, FITC-dextran, Alexa680-dextran', stepSizeX:'0.58 um, 1.16 um', stepSizeY:'0.58 um, 1.16 um', stepSizeZ:'5 um'迈特沃克斯（The MathWorks）公司的Matlab数据文件（后缀为.mat）以皮层深度命名，内含Oxyphor2P衰减数据（标识为'Oxyphor2P.decay'）、经其衍生计算得到的氧分压（pO2）数值（'Oxyphor2P.pO2Value'）及其对应坐标：X轴坐标为'Oxyphor2P.X_reference_coordinates'，Y轴坐标为'Oxyphor2P.Y_reference_coordinates'。上述数据关联以下三类参考图像：经SR101染色的星形胶质细胞图像（'reference.SR101'）、标记有荧光素异硫氰酸酯（FITC）的血管图像（'reference.FITC'）以及Oxyphor2P分布图像（'reference.Oxyphor2P'）。 数据集元数据：标题为"Baseline oxygen consumption decreases with cortical depth: Artery02_010819_VG674"，其他说明：磷光寿命氧成像技术。 标本元数据：本地标识符：VG674；物种：小鼠；NCBI分类号：NCBI:txid10090；年龄：212.0；年龄单位：日；性别：雌性；基因型：C57BL6；器官名称：大脑；样本本地标识符：Artery02_010819_VG674；采样部位：躯体感觉皮层。 仪器元数据：显微镜类型：双光子激光扫描显微镜；显微镜制造商及型号：布鲁克（Bruker）Ultima；物镜型号：奥林巴斯UMPlanFL N、奥林巴斯XLUMPlanFl；物镜浸液：水浸式；物镜数值孔径：0.5、0.95；物镜放大倍数：20倍；探测器类型：光电倍增管（PMT）；照明光源：相干（Coherent）公司Chameleon Ultra飞秒钛宝石激光器。 图像元数据：X轴方向：后-前；Y轴方向：左-右；Z轴方向：上-下；通道编号：1、2，对应红、绿通道；显示颜色：(1.0, 0.0, 0.0)、(0.0, 1.0, 0.0)；通道说明：1对应Oxyphor 2P信号，2对应FITC信号；荧光标记物：Oxyphor2P、FITC-葡聚糖、Alexa680-葡聚糖；X轴步长：0.58 μm、1.16 μm；Y轴步长：0.58 μm、1.16 μm；Z轴步长：5 μm。