CCALNN-PEG monolayer proportion influence on gold nanoparticles uptake (Figure S3 dataset)

HeLa cells were incubated in suspension with 5 nm diameter gold nanoparticles (final concentration 100 nM) coated with a mix of CALNN and CCALNN-PEG peptides for 10 min in serum-free medium and a further 30 min in complete medium (10 % FCS). Nanoparticles/medium were then discarded, cells transferred to a dish and left to attach for 4 h in complete medium, fixed and later imaged by photothermal microscopy. Images are two-plane TIFF images of the nanoparticles uptake by HeLa cells. The first image plane is the brightfield image, and the second image plane is the photothermal image. 0%_CCALNN-PEG_&_100%_CALNN_[1-5].tif are images of cells incubated with nanoparticles coated with 100% CALNN peptides. 10%_CCALNN-PEG_&_90%_CALNN_[1-4].tif are images of cells incubated with nanoparticles coated with 90% CALNN and 10% CCALNN–PEG peptides. 20%_CCALNN-PEG_&_80%_CALNN_[1-4].tif are images of cells incubated with nanoparticles coated with 80% CALNN and 20% CCALNN–PEG peptides. 30%_CCALNN-PEG_&_70%_CALNN_[1-5].tif are images of cells incubated with nanoparticles coated with 70% CALNN and 30% CCALNN–PEG peptides.将处于悬浮状态的海拉细胞（HeLa）与粒径5 nm、经CALNN与CCALNN-PEG多肽混合包被的金纳米颗粒（终浓度100 nM）置于无血清培养基中孵育10分钟，随后转移至含10%胎牛血清（FCS）的完全培养基中继续孵育30分钟。随后弃去纳米颗粒与培养基的混合液，将细胞转移至培养皿中并于完全培养基中静置贴壁4小时，固定细胞后通过光热显微镜（photothermal microscopy）成像。所获图像为海拉细胞摄取纳米颗粒的双平面TIFF图像，其中第一图像平面为明场图像，第二图像平面为光热图像。0%_CCALNN-PEG_&_100%_CALNN_[1-5].tif为使用100% CALNN多肽包被的纳米颗粒孵育的细胞样本图像；10%_CCALNN-PEG_&_90%_CALNN_[1-4].tif为使用90% CALNN与10% CCALNN-PEG多肽包被的纳米颗粒孵育的细胞样本图像；20%_CCALNN-PEG_&_80%_CALNN_[1-4].tif为使用80% CALNN与20% CCALNN-PEG多肽包被的纳米颗粒孵育的细胞样本图像；30%_CCALNN-PEG_&_70%_CALNN_[1-5].tif为使用70% CALNN与30% CCALNN-PEG多肽包被的纳米颗粒孵育的细胞样本图像。