Gene expression analysis of immortalized human liver progenitor-like cells in culture

We described a highly efficient method to establish the immortalized HepLPC cell, these cell lines could efficiently expand and able to readily differentiate back into metabolically functional hepatocytes. To further characterize them, we compare the global expression profiles among immortalized human liver progenitor-like cells (iHepLPCs), hepatocyte-like cells differentiated from iHepLPCs (iHepLPCs-Hep) and 3D cell spheroids of the iHepLPCs-Hep (iHepLPCs-Hep-3D). Immortalized human liver progenitor-like cells (iHepLPCs) were cultured in the transition and expansion medium or in the modified hepatic maturation medium for 9 days. 3D cell spheroids of the iHepLPCs-Hep was aggregated by iHepLPCs and then cultured in the modified hepatic maturation medium for another 9 days. Sample was assayed in double.