Figure 5 Dataset - Monocyte circulation facilitates increased transmigration and adhesion compared to static conditions

The dataset includes raw confocal images of live-labeled monocytes cultured with or without circulating flow and in the absence or presence of a chemoattractant protein in a microfluidic device. The prism file includes the final figure outline as well as the raw quantification expression values.The images can be analyzed in a software that enables 3D image analysis, such as Imaris. To quantify fluorescently labeled monocytes in confocal images, the Spots tool in Imaris was used to identify cells based on a specified diameter (10 um). Cells below the membrane were considered to be transmigrated. A fluorescent intensity threshold was determined and kept consistent across images for Spot identification. Quantified data was exported from Imaris and analyzed in GraphPad Prism.This dataset supports Fluid flow impacts endothelial-monocyte interactions in a model of vascular inflammatory fibrosis, published in <i>Scientific Reports</i><i>,</i><i> </i>Vol. 15, Iss. 3227, January 2025.