Potential of Insect Vector Screening Method for Development of Durable Resistance Cultivars to Rice yellow mottle virus Disease

The study aimed to investigate the potential of insect vector Rice yellow mottle virus (RYMV) cultivar screening method. Screening rice cultivars against RYMV under artificial conditions is usually carried out inside the screen house by mechanical inoculation of RYMV isolates. Such an approach may be highly criticized as not fully representative of how RYMV disease is spread or transmitted under field conditions. Consequently, the potential of three RYMV insect vectors, Oxya hyla, Locris rubra and chnootriba similes, was evaluated in comparing the cultivar screening method with mechanical transmission using eight differential rice genotypes against a highly virulent RYMV Nigerian isolate. The study revealed that each of the three insect vector methods is different from the mechanical transmission method and all methods screened rice cultivars in the same way. This study revealed the potential of the insect vector screening method to provide a basis not only for the development of durable resistant cultivars to RYMV disease but also for further investigation on vectors, virus and plants interaction.本研究旨在探究昆虫介体介导的水稻黄斑驳病毒（Rice yellow mottle virus, RYMV）品种筛选方法的应用潜力。当前人工条件下抗RYMV水稻品种的筛选，通常是在防虫网室内通过机械接种RYMV分离物来完成的，但该方法常因无法完全反映田间条件下RYMV病害的自然传播规律而饱受诟病。为此，本研究选取3种RYMV昆虫介体——Oxya hyla、Locris rubra与Chnootriba similes，以8个鉴别性水稻基因型为材料，对接尼日利亚高毒力RYMV分离物，对比评估昆虫介体接种法与机械接种法在品种筛选中的应用效果。研究结果表明，三种昆虫介体接种法均与机械接种法存在差异，但所有接种方式对水稻品种的筛选结果均保持一致。本研究证实，昆虫介体筛选方法不仅可为培育抗RYMV病害的持久抗性品种提供理论支撑，也为后续开展介体-病毒-植物互作相关研究奠定了坚实基础。