<i>dPNUTS</i> mutant clones reveal a cell autonomous growth defect in developing tissues.

Clones (marked by absence of GFP) of either wild-type or dPNUTS13B mutant cells are shown in wing imaginal discs obtained from 3rd instar larvae. Clones were induced in a wild type (A–J) or Minute (M) mutant background (K–R) 48 hr, 72 hr or 96 hr prior to dissection, as indicated. The parental (p) genotypes are indicated, along with the genotype of clones (c) generated by FLP-mediated mitotic recombination and are coded with grayscale to indicate the relative level of GFP expression. A–J, wing discs were stained for activated caspase shown in blue, and in cross sections (E–J), apico-lateral junctions are marked by discs-large staining in red; GFP is shown in green. In cross sections Q–R, DNA is shown in red. Arrowheads in panel L indicate the presence of dPNUTS13B GFP-negative clones in a Minute (M) mutant background. +, M, GFP/+, M, GFP twinspot clones, indicated by arrowhead in panel R, were almost never observed because of a severe growth defect.