Macrosystems Results for ITS in 7 Plot Soil Samples (UPARSE - Resampled)

Patterns of biodiversity, such as the increase toward the tropics and the peaked curve during ecological succession, are fundamental phenomena for ecology. Such patterns have multiple, interacting causes, but temperature emerges as a dominant factor across organisms from microbes to trees and mammals, and across terrestrial, marine, and freshwater environments. However, there is little consensus on the underlying mechanisms, even as global temperatures increase and the need to predict their effects becomes more pressing. The purpose of this project is to generate and test theory for how temperature impacts biodiversity through its effect on biochemical processes and metabolic rate. A combination of standardized surveys in the field and controlled experiments in the field and laboratory measure diversity of three taxa -- trees, invertebrates, and microbes -- and key biogeochemical processes of decomposition in seven forests distributed along a geographic gradient of increasing temperature from cold temperate to warm tropical. This data set captures abundance of OTUs (Operational Taxonomic Units) sampled for in forest soils at the original six sampling sites (HJ Andrews, Niwot, Harvard Forest, Coweeta, Luquillo, Barro Colorado Island) plus the new site set up by the Enquist Lab at Mt. Bigelow near Tucson, AZ. This data represents abundance of ITS (fungi) genes in soil samples processed by the University of Oklahoma Institute for Environmental Genomics as part of a macrosystems biodiversity and latitude project supported by the National Science Foundation under Cooperative Agreement DEB#1065836.生物多样性（biodiversity）格局——诸如向热带地区递增的分布规律，以及生态演替（ecological succession）过程中的峰值曲线——是生态学（ecology）的核心基础现象。这类格局存在多种相互作用的成因，但温度被证实为支配从微生物、树木到哺乳动物各类生物，以及陆地、海洋与淡水生境的主导影响因子。然而，尽管全球气温持续升高，预测其生态效应的需求愈发紧迫，学界对其背后的作用机制仍未达成广泛共识。本项目旨在构建并检验相关理论，以阐明温度如何通过影响生化过程与代谢速率，进而对生物多样性产生作用。研究结合野外标准化调查与野外、实验室受控实验，对沿从寒温带到暖热带的温度地理梯度分布的7片森林中的3类类群——树木、无脊椎动物与微生物——的多样性，以及关键的生物地球化学分解过程进行了测定。本数据集涵盖了原始6个采样点（HJ Andrews、Niwot、Harvard Forest、Coweeta、Luquillo、Barro Colorado Island）以及由Enquist实验室在亚利桑那州图森附近的Bigelow山新建采样点的森林土壤中采样得到的OTUs（操作分类单元，Operational Taxonomic Units）丰度数据。该数据为土壤样本中的真菌ITS（内转录间隔区，Internal Transcribed Spacer）基因丰度数据，相关研究属于美国国家科学基金会以合作协议DEB#1065836资助的大尺度生物多样性与纬度梯度项目的一部分，土壤样本由俄克拉荷马大学环境基因组学研究所完成处理。