遇见数据集

Transcriptome coverage of the mm10 mouse reference genome across 200bp windows in ten closely related taxa and three tissues.

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DataONE2016-02-12 更新2024-06-27 收录
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Transcriptome coverage of the mm10 mouse reference genome. Computed from transcriptome alignments of three populations of Mus musculus domesticus (AH from Iran, CB from Germany, MC from France), two populations of Mus musculus musculus (KH from Kazakhstan, WI from Austria), Mus musculus castaneus (TA), Mus spicilegus (SC), Mus spretus (SP), Mus mattheyi (MA) and Apodemus uralensis (AP). The first six fields correspond to the genomic location and the following each of the transcriptomes of the species mentioned here. Brain samples (pbrain), liver samples (pliver) and testis samples (ptestis), correspond to sequencing done at approximately one third of an Illumina HiSeq 2000 lane per taxon, while additional brain samples (xbrain) were done in a whole illumina HiSeq 2000 lane per taxon. Features were generated with bedtools, converted into SAF format, and extracted from BAM alignments using the featureCounts suite.

本数据集为基于mm10小鼠参考基因组的转录组覆盖度数据,其计算基于以下类群的转录组比对结果:3个小家鼠家栖亚种(Mus musculus domesticus)种群(AH样本源自伊朗、CB样本源自德国、MC样本源自法国)、2个小家鼠指名亚种(Mus musculus musculus)种群(KH样本源自哈萨克斯坦、WI样本源自奥地利),以及小家鼠印度亚种(Mus musculus castaneus,TA)、尖毛鼠(Mus spicilegus,SC)、西班牙鼠(Mus spretus,SP)、马蒂鼠(Mus mattheyi,MA)及乌拉尔姬鼠(Apodemus uralensis,AP)。数据集前六个字段对应基因组位置,后续字段依次对应上述各物种的转录组数据。脑组织样本(pbrain)、肝脏组织样本(pliver)与睾丸组织样本(ptestis)的每个类群测序通量约占一条Illumina HiSeq 2000测序通道的三分之一;额外脑组织样本(xbrain)的每个类群测序通量则占完整一条Illumina HiSeq 2000测序通道。转录组特征通过bedtools工具(bedtools)生成并转换为SAF格式(SAF format),随后借助featureCounts工具套件(featureCounts suite)从BAM比对文件(BAM alignments)中提取得到。

创建时间:
2016-02-12
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