Metagenomic, metatranscriptomic, and single cell sequencing data from an Environmental Sample Processor deployment in Monterey Bay, CA in 2016.

<p>These metagenomic and metatranscriptomic time-series data cover a 52-day period in the fall of 2016 during an intense bloom of the dinoflagellate Akashiwo sanguinea in Monterey Bay, CA, USA. The dataset comprises 84 metagenomes, 82 metatranscriptomes, and 88 16S rRNA amplicon libraries that capture the functions and taxonomy the bacterial and archaeal community. In addition, 88 18S rRNA amplicon libraries describe the taxonomy of the eukaryotic community during the bloom. Microbial cells were collected at station M0 using the moored autonomous robotic Environmental Sample Processor (ESP) instrument and preserved with RNAlater in the instrument until retrieval.</p>本宏基因组（metagenomic）与宏转录组（metatranscriptomic）时间序列数据集覆盖了2016年秋季美国加利福尼亚州蒙特雷湾发生血红哈卡藻（Akashiwo sanguinea）大规模水华期间的52天观测周期。该数据集包含84个宏基因组样本、82个宏转录组样本以及88套16S rRNA扩增子文库，可用于解析细菌与古菌群落的功能及分类学特征；此外，配套的88套18S rRNA扩增子文库可描述此次水华期间真核生物群落的分类学组成。研究人员借助锚定式自主机器人环境样品处理器（Environmental Sample Processor，ESP）在M0站位采集微生物细胞，并在仪器内使用RNAlater试剂对样品进行保存，直至样品回收。